Homemade Chemiluminescent Protocols?

HK hk-miami at ix.netcom.com
Wed Jun 4 19:01:15 EST 1997

x-no-archive: yes
In <5n47to$188 at falcon.le.ac.uk> "Dr E. Buxbaum" <EB15 at le.ac.uk> writes:
>hk-miami at ix.netcom.com(HK) wrote:
>>Maybe it wasn't the luminol...Maybe you mixed or measured something
>>incorrectly, since you said that you only tried it once.
>>I buy Luminol from Sigma...it works just fine.  In a side-by-side
>>test of my solutions and the ones in the  Amersham ECL kit, my
>>solutions work equally as well.
>I would not say things like this if I hadn't done the ovious tests. In
>general I had quite a few problems with Sigma's chemicals over the
>for example SDS which was insoluble in water, inactive enzyme... Looks
>like a quality control problem to me. 
I'm not sure what a more oBvious test would be than testing it side by
side with the reagents I was trying to duplicate and seeing that both
worked equally well.  What other test would you suggest?


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