Yeast artificial chromosome

Andrei Popov andrei.popov at bbsrc.ac.uk-x
Tue Jun 24 16:51:30 EST 1997


Bryan Moore wrote:
> 
> Does anyone have a reliable method for transfecting a YAC into tissue culture cells besides calcium
> phosphate, electroporation, or lipofectin amine??
> 
> Bryan Moore
> 
> University of Alabama at Birmingham
> Department of Biochemistry and Molecular Genetics
> (205) 975-8824

from the above 3 methods works only 1- lipofection.

Also people microinject YACs- up to 600 kb,
and use mammalian cell/yeast spheroplast fusion

(Pachnis et al 
PNAS 1990 
v87 5109-13)

best luck :-)

-- 
Andrei Popov

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