ANNOUNCEMENT: EMBO Practical Course - Microinjection, Electroporation and Particle Transfection of Cells

Rainer Saffrich saffrich at
Fri Mar 7 13:17:58 EST 1997

EMBO Practical Course
Microinjection, Electroporation and Particle Transfection of Cells

EMBL Heidelberg, Germany    
May 26 - 31, 1997

The aim of the course is to teach all aspects of the capillary
microinjection, electrotransfection and particle transfection
techniques of cells, including the automated systems. 
Methods and applications will be discussed and extensively practised
or demonstrated in the laboratory. Lectures and seminars will round up
the program. The course is intended mainly for Ph.D. students and
postdoctoral scientists. The selection of participants will be 
on the basis of their scientific background, the importance of the
course techniques for their own research and according to the criteria
implied by EMBO policy.

Practical laboratory course program includes i.e.

-  Nuclear and cytoplasmic injection of fluorescent dyes and tracers
-  Nuclear microinjection of DNA
-  Cytoplasmic microinjection of antibodies
-  Injection of plant cells
-  Injection of Xenopus oocytes
-  Injections with an automated system
-  Analysis by immunfluorescence techniques
-  Evaluation of experiments and analysis of results by fluorescence
    microscopy and image  processing
-  Gene transfer by electroporation into pro- and eucaryotic cells
-  Gene transfer with particle bombardment

The following applications of the techniques will be discussed: DNA
transfer, mRNA expression, microcloning, characterisation of
subnuclear components, Xenopus oocytes systems, transfer of genes in
fertilised eggs, other methods for transfer of biological material 
into cells. There will be lectures on special topics from invited
speakers, blackboard sessions for the practicals and demonstrations
and presentations of the participants about their own field of
interest and research. The course consists of about 75% practical work
and 25% lectures and seminars.

Participants may bring their own material (cells, samples, probes,...)
to the course and attempt to work with it and begin to try solving
their own problems with the introduced techniques.

For application to the course no spcial form is needed. Please just
send a formal letter including a short c.v., statement about your
current research and recent publications before April 1st, 1997 to: 

Dr. W. Ansorge
Meyerhofstr. 1
D-69117 Heidelberg

Telephone:	+49-6221-387355 or +49-6221-387535
Telefax:	+49-6221-387306

Lodging and meals during the course are paid by EMBO. The registration
fee is DM 200,- but may be waived under special circumstances. The
number of participants is limited to 16. For further information and
questions about the course please contact us again.

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