293 cells with transformed phenotype?
Ian A. York
iayork at panix.com
Tue Mar 18 10:23:38 EST 1997
In article <graham-1803971207460001 at tastiger.dbe.csiro.au>,
Lloyd Graham <graham at dbe.csiro.au> wrote:
>At ATCC+12 passages, my 293 cells (HEK293) have spontaneously changed
>phenotype to poorly adhered cells that grow in clumps, rather as if they
>have undergone a spontaneous transformation. All I did was subculture them
Have you checked for mycoplasma? I've not seen this happen with any of
the 293 I've carried.
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
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