293 cells with transformed phenotype?
graham at dbe.csiro.au
Mon Mar 17 20:01:28 EST 1997
At ATCC+12 passages, my 293 cells (HEK293) have spontaneously changed
phenotype to poorly adhered cells that grow in clumps, rather as if they
have undergone a spontaneous transformation. All I did was subculture them
in a standard way.
I have just made a series of stable 293 transfectants (starting with cells
of normal phenotype) and they too had switched to the novel phenotype by
the end of the selection procedure.
Does anyone know what's going on with these cells?
Dr. Lloyd Graham
CSIRO Div. Biomolecular Engineering,
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