QE: nuclear extracts

[Dr.Tilman Voss]

Lloyd Graham wrote:
> 
> At ATCC+12 passages, my 293 cells (HEK293) have spontaneously changed
> phenotype to poorly adhered cells that grow in clumps, rather as if they
> have undergone a spontaneous transformation. All I did was subculture them
> in a standard way.
> 
> I have just made a series of stable 293 transfectants (starting with cells
What is thew best method for extracting nuclear proteins. I want to
separate them on 2D gels.
Thanks
Tilman Voss
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Boehringer-Ingelheim-Research Vienna