Yeast nucleus staining
martin.ligr at po.uni-stuttgart.de
Mon Mar 24 09:22:43 EST 1997
I am currently using DAPI to visualize yeast nuclei, but the signal from
nucleus is often obscured by strong signal from mitochondria. So I am
thinking about using Hoechst 33258 or 33342. Could someone give me some
info what is the difference between these two and how do they compare to
DAPI? And is there any nucleus specific stain at all?
P.S.: Please respond by email if possible, our news server has a rather
erratic performance. If there is enough interest in this topic, I will
post a summary.
Institut fuer Biochemie
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