elisaproblems

at sigmatau.it at sigmatau.it
Mon Mar 24 10:19:04 EST 1997


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@sigmatau.it wrote:
> 
> I am trying to optimalize a self-made elisa that detects IgG`s in the
> supernatant of a particular cell-line. I coat with anti-IgG and develop
> with GPOD. The Elisa is not bad, but sometimes there is too much
> background. Can anyone give me some hints to reduce the background
> values???
> 
> Did you try with detergents in the washings and in the incubation mix?
> (0.5% Tween 20)

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Date: Mon, 24 Mar 1997 16:12:35 +0100
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I am trying to optimalize a self-made elisa that detects IgG`s in the
supernatant of a particular cell-line. I coat with anti-IgG and develop
with GPOD. The Elisa is not bad, but sometimes there is too much
background. Can anyone give me some hints to reduce the background
values??? 


Did you try with detergents in the washings and in the incubation mix?
(0.5% Tween 20)


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