Nuclear staining

[Martin Ligr]

Hello:
I am currently using DAPI to visualize yeast nuclei, but the signal from
nucleus is often obscured by strong signal from mitochondria. So I am
thinking about using Hoechst 33258 or 33342. Could someone give me some
info what is the difference between these two and how do they compare to
DAPI? And is there any nucleus specific stain at all?
Thanks,
Martin Ligr

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Martin Ligr
Institut fuer Biochemie
Universitaet Stuttgart
Pfaffenwaldring 55
D-70569 Stuttgart
Germany

phone:	+49-(0711)-685-4383
fax:	+49-(0711)-685-4392