Yeast nucleus staining

Michael Lichten lichten at
Tue Mar 25 09:18:47 EST 1997

In article <33368E33.41C6 at>, Martin Ligr
<martin.ligr at> wrote:

> Hello:
> I am currently using DAPI to visualize yeast nuclei, but the signal from
> nucleus is often obscured by strong signal from mitochondria. So I am
> thinking about using Hoechst 33258 or 33342. Could someone give me some
> info what is the difference between these two and how do they compare to
> DAPI? And is there any nucleus specific stain at all?
> Thanks,
> Martin Ligr

I believe that all, unfortunately, preferentially stain AT-rich sequences,
which is why the high mitochondrial staining.  You may wish to try
propidium iodide, which in our hands gave much less mitochondrial

Michael Lichten
lichten at

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