ANNOUNCEMENT: EMBO PRACTICAL COURSE - Microinjection, Electroporation and Particle Transfection of Cells

Rainer Saffrich saffrich at
Sat Mar 29 15:55:24 EST 1997


Electroporation and 
Particle Transfection of Cells

European Molecular Biology Laboratory
Heidelberg, Germany

May 26 - 31, 1997

W. Ansorge, G. Neuhaus, R. Saffrich, M.F. Trendelenburg 

Speakers and Instructors: 
W. Ansorge (EMBL), W. Blickle (Koeln), D. Brocksch (Jena), J.E. Celis
(Aarhus), A. Ephrussi (EMBL), R. Fischer (Heidelberg), T. Hyman
(EMBL), I. Mattaj (EMBL), G. Neuhaus (Freiburg),
R. Pepperkok (London), R. Saffrich (EMBL), E. Stelzer (EMBL), M.F.
Trendelenburg (Heidelberg)

The aim of the course is to teach all aspects of the capillary
microinjection, electrotransfection and particle transfection
techniques of cells, including the automated systems. Methods and
applications will be discussed and extensively practised or
demonstrated in the laboratory. Lectures and seminars will round up
the program. The course is intended mainly for Ph.D. students and
postdoctoral scientists. The selection of participants will be on the
basis of their scientific background, the importance of the course
techniques for their own research and according to the criteria
implied by EMBO policy.

Practical laboratory course program includes:

· Nuclear and cytoplasmic injection of fluorescent dyes and tracers
· Nuclear microinjection of DNA
· Cytoplasmic microinjection of antibodies
· Injection of plant cells
· Injection of Xenopus oocytes
· Injections with an automated system
· Analysis by immunofluorescence techniques
· Evaluation of experiments and analysis of results by fluorescence 
  microscopy and image processing
· Gene transfer by electroporation into pro- and eucaryotic cells
· Gene transfer with particle bombardment

The following applications of the techniques will be discussed: DNA
transfer, mRNA expression, microcloning, characterisation of
subnuclear components, Xenopus oocytes systems, transfer of genes in
fertilised eggs, other methods for transfer of biological material
into cells. 
There will be lectures on special topics from invited speakers,
blackboard sessions for the practicals and demonstrations and
presentations of the participants about their own field of interest
and research. 
The course consists of about 75% practical work and 25% lectures and

Participants may bring their own material (cells, samples, probes,...)
to the course and attempt to work with it and begin to try solving
their own problems with the introduced techniques.

For application to the course no special form is required. To apply,
please send just a formal letter including a short c.v., a statement
about your current research and a list of recent publications to:

Dr. W. Ansorge
Meyerhofstr. 1
D-69117 Heidelberg

Telephone:	+49-6221-387355 or +49-6221-387535
Telefax:		+49-6221-387306
E-Mail:		saffrich at

Deadline for applications is April 1st, 1997.

Lodging and meals during the course are paid by EMBO. The registration
fee is DM 200,- but may be waived under special circumstances. The
number of participants is limited to 16. For further information and
questions about the course please contact us again.

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