FACS Sensitivity

Jumbuck jumbuck at usa.net
Thu May 8 01:30:32 EST 1997


Martin Offterdinger wrote:
> 
> On Fri, 2 May 1997 13:21:21 +0100 (BST), BMBRNL at leeds.ac.uk (R.N.
> Leach) wrote:
> 
> >Could anyone tell me the limit of sensitivity of fluorescence activated cell
> >sorting? Is it possible to detect, for example,one labelled cell out of a
> >population of 1 million cells?
> >Thank you in advance.
> >Rob Leach,Ph.D.,
> >Dept. of Biochemistry and Molecular Biology,
> >University of Leeds
> Theoretically it is, but
> 1) You will need enourmous amounts of cells, to achieve statistical
> significance-in your case at least 1000 million cells.
> 2)the experiment will take quite a lot of time, because you have to
> pass these 1000million cells through the FACScan!
> 
> hope this helps!
> Martin


what if all your intereseted in is the positive/negative aspect of the 
marking, rather than the level of positivity (flourescence) per cell?

Are any other antibody related assays more sensitive?

Robert
University of Melbourne



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