Sonication with PMSF/EGTA produces a precipitate.....why?

Doug Hubatsch Doug.Hubatsch at arcm.ca.astra.com
Mon Nov 3 17:31:10 EST 1997


Anybody else come across this?  When I sonicate bacteria in .1 mM PMSF/10
mM EDTA, add 1% SDS and incubate for 30 min. at RT I get a DNA-like
precipitate.  I don't think its because of the SDS, boiling the mixture
doesn't put the precipitate back into solution.  The concentrations for
PMSF and EDTA are well below other reported concentrations so I don't
really have any idea what could be causing it.

Any ideas?

Doug



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