mouse serum for cell culture

Andrei Popov andrei.popov at SPAM.bbsrc.ac.uk
Mon Nov 3 16:35:58 EST 1997


stefan schnell wrote:
> 
> hello,
> I am interested in growing murine bone-marrow cells in
> cytokine-conidtioned medium using serum from the same mouse as serum
> source. Mouse serum seems to be inhibitory

why should the serum be good for the bone marrow cells?
Most cytokines are made locally and used locally and do not
appear in active concentrations in the peripheral blood.

, anny suggestions how to
> improve the quality? Is there anybody selling mouse serum for cell
> culture use. 

about 5-6 years ago there was a paper where people used
the mouse serum to culture murine hybridoma cells after fusion
and they claimed the improvement in the hybridoma clone
frequency.
 
best regards,

-- 
Andrei Popov

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