proteinase inhibitors

Jan enghi001 at mc.duke.edu
Tue Nov 4 22:45:11 EST 1997


Hi,

We are using a pulse-chase protocol to study the biosynthesis of a
protein in HUVEC cells. We would like to inhibit a specific
intracellular processing proteinases. This proteinase cleaves a basic
(lots of Arg and Lys residues) region in the protein. We plan to add
proteinase inhibitor(s) to the cell culture medium to abolish this
processing event.

The question is which proteinase inhibitors have previously been
employed in similar studies.

Thank in advance

Jan






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