measure superoxide dismutase activity in a flowcytometer

Eli Hestermann ehestermann at whoi.edu
Thu Nov 13 14:36:47 EST 1997


There are a couple fluorescent probes that recognize reactive oxygen
species, i.e. hydroethidine and dichlorofluorescein diacetate.  Their
specificity for superoxide vs. peroxide has been questioned, though.  It
seems to me that a protocol could be developed using these probes.
Check out Meth. Cell Biol., volume 41, chapter 28 for more details.

Eli
--
Eli V. Hestermann
ehestermann at whoi.edu
http://www.mit.edu/people/octavian/eli.html
"Vita brevis est, ars longa" - Seneca





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