looking for info on ELISA test

Eric1963 eric1963 at aol.com
Thu Nov 20 21:24:51 EST 1997


The enzyme linked immunosorbant assay (ELISA) is based on the specificity of
immunoglobulins (antibodies).  The sandwich ElISA is the most common approach,
antibodies are adsorbed to a substrate (usually a 96 well plate), the analyte
(test substance - a protein) is then added, followed by the addition of an
antibody conjugated to an easily detectable molecule such as fluorscein
isothiocynate (FITC), a radiolabel or a colorometric product.  The advantage to
ELISA is that a complex biological mixture can be assayed for a specific
protein without the need to clean up the sample.  You can find more info on
ELISA in any Microbiology or Cell Biology text.  There are many companies which
supply ELISA kits to assay many different proteins.  I hope this answers some
of your questions.
 



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