Deglycosylation of N-linked and O-linked Oligosaccharides

[DOCTORHIM at aol.com]

A number of you have enquired about deglycosylating proteins of both N and O
linked oligosaccharides. 

I think the kit that you are looking for is made by Prozyme.  It has five
enzymes that will remove virtually all N and O linked oligosaccharides.  The
instruction book is a good reference on techniques and specificities of the
enzymes and comparison with chemical methods. 

You can download the Instructions for this kit at:
http://www.prozyme.com/glycoenzymes/

You will need Adobe Acrobat 3 reader to view it.  It is also available for
download at this site but it may take 30-60 minutes depending on your modem
speed. 

Good luck!