HOT RT-PCR?

Barbi bgould at PO-BOX.MCGILL.CA
Fri Oct 24 10:09:04 EST 1997


Does anyone have any suggestions on how to get rid of background
radioactivity from hot RT-PCR?  There is a long, dark smear above my bands
regardless of how much RNA I start with.  I'm running my PCR products in a
4% acrylamide gel.

Thanks

Barbi





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