Hendel hendel at
Sun Oct 26 14:38:10 EST 1997

bgould at PO-BOX.MCGILL.CA (Barbi) wrote:

>Does anyone have any suggestions on how to get rid of background
>radioactivity from hot RT-PCR?  There is a long, dark smear above my bands
>regardless of how much RNA I start with.  I'm running my PCR products in a
>4% acrylamide gel.

You can try one of the comercially available spin-columns (chroma-spin
or some such). They remove anything below a certain size limit. Once
upon a time, when I was doing HOT-PCR I used this technique.

Good luck

    Jakob Hendel
Dept.of Gastroenterology
    Kas Glostrup
Phone: +45 43232806
Fax:   +45 43232301
e-mail: hendel at

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