hendel at post4.tele.dk
Sun Oct 26 14:38:10 EST 1997
bgould at PO-BOX.MCGILL.CA (Barbi) wrote:
>Does anyone have any suggestions on how to get rid of background
>radioactivity from hot RT-PCR? There is a long, dark smear above my bands
>regardless of how much RNA I start with. I'm running my PCR products in a
>4% acrylamide gel.
You can try one of the comercially available spin-columns (chroma-spin
or some such). They remove anything below a certain size limit. Once
upon a time, when I was doing HOT-PCR I used this technique.
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