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Help for bovine aortic smooth muscle cell culture

Dan Johnson adj8n at virginia.edu
Fri Oct 31 11:56:07 EST 1997


In article <63a4e2$12i2 at hkusud.hku.hk>, "Michael Koon"
<hwkoon at hkusua.hku.hk> wrote:

> Hello.
> 
> I need to culture bovine aortic smooth muscle cell.
> 
> I do not know what medium should be used.
> 
> What supplements or growth factors are required?
> In how much in amount?
> 
> Please give me some help. Thanks in advance.\
> 
> From Michael Koon

Michael:

					They are relatively easy to grow, compared to other cell types.
I have grown them successfully in DMEM, alone or mixed 1:1 with M199, and
supplemented with 10% fetal calf serum, penecillin and streptomycin.  No
other specific growth factors need be be added.

Good luck.  Dan Johnson



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