In article <63a4e2$12i2 at hkusud.hku.hk>, "Michael Koon"
<hwkoon at hkusua.hku.hk> wrote:
>> I need to culture bovine aortic smooth muscle cell.
>> I do not know what medium should be used.
>> What supplements or growth factors are required?
> In how much in amount?
>> Please give me some help. Thanks in advance.\
>> From Michael Koon
They are relatively easy to grow, compared to other cell types.
I have grown them successfully in DMEM, alone or mixed 1:1 with M199, and
supplemented with 10% fetal calf serum, penecillin and streptomycin. No
other specific growth factors need be be added.
Good luck. Dan Johnson