Problem with Neuronal Culture

Marco Malgarini marco at
Tue Sep 2 10:43:35 EST 1997

Jerome Freed wrote:
> Bill Marszalec wrote:
> >    I would like to pose a problem to those members of this group who
> > have worked with cultured neurons or cultured cells in general.  For the
> > past three years, we have been performing whole cell voltage clamp
> > experiments using a primary culture of isolated frontal cortical neurons
> > prepared from 17 day embryonic rat pups.  My question relates to a
> > seemingly cyclic fluctuation of the quality of these cells.
> ----------------------
> It would be very prudent to test your cultures (good and bad) for
> mycoplasma.  The method of fluorescent staining of 3T3 cell cultures
> inoculated with some of the medium from your neuronal cultures, using a
> DNA stain like Hoechst or DAPI, would be the simplest to carry out.
> Further, is it possible to passage the evil influence (or putative
> agent) to some other cultures that seem normal and transfer the
> cytopathology you have observed?

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