monensin in cell culture

Ross Beck r.beck at unsw.edu.au
Tue Sep 9 23:32:28 EST 1997


Hi, I wish to grow up some THP-1 (monocytic) cells, which I plan to hit 
with LPS so that they produce IL-8. But I want it stored 
intra-cellularly, not secreted. I've heard that monensin can be used for 
this sort of thing. Can anyone supply an expert opinion?



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