Question about Immunoprecipitation

[jnbai]

Hi, everyone:

I am planning to IP a nuclear protein. However, generally IP protocol
suggest to lyse the cell, follow by a low speed spin and use the
supernatant for IP.

My question is:
1) After the lysis, does this low speed spin get rid of the nucleus?
2) Is there a way to retrieve as much nuclear protein as possible
through the lysis?

Thanks in advance.

Jining
jnbai at welchlink.welch.jhu.edu