Question about Immunoprecipitation

jnbai jnbai at WELCHLINK.WELCH.JHU.EDU
Thu Sep 18 00:27:20 EST 1997


Hi, everyone:

I am planning to IP a nuclear protein. However, generally IP protocol
suggest to lyse the cell, follow by a low speed spin and use the
supernatant for IP.

My question is:
1) After the lysis, does this low speed spin get rid of the nucleus?
2) Is there a way to retrieve as much nuclear protein as possible
through the lysis?

Thanks in advance.

Jining
jnbai at welchlink.welch.jhu.edu



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