How to deplete Oxygen from enzyme assay?
William P. Tschantz
tschantz at galactose.mc.duke.edu
Fri Sep 19 12:52:51 EST 1997
I am looking for an easy way to deplete a system of oxygen. I suspect
that the enzyme i am working on uses molecular oxygen in its mechanism and
would like to test it. I have tried degassing my samples and reaction
vial with nitrogen, but have gotten messy results. Enzyme linked assays
would be the easiest i think.
Any ideas appreciated.
Thanks in advance.
| William R. Tschantz, Ph.D. * Drink Real Beer |
| Dept of Pharm and Cancer Biology* Have a Homebrew! |
| Duke University, Durham, NC * It's Good for you! |
| tschantz at galactose.mc.duke.edu * |
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