How to deplete Oxygen from enzyme assay?

William P. Tschantz tschantz at galactose.mc.duke.edu
Fri Sep 19 12:52:51 EST 1997


Hi

I am looking for an easy way to deplete a system of oxygen.  I suspect
that the enzyme i am working on uses molecular oxygen in its mechanism and
would like to test it.  I have tried degassing my samples and reaction
vial with nitrogen, but have gotten messy results.  Enzyme linked assays
would be the easiest i think.

Any ideas appreciated.

Thanks in advance.

Bill
-- 
| William R. Tschantz, Ph.D.       * Drink Real Beer                  |
| Dept of Pharm and  Cancer Biology*        Have a Homebrew!          |
| Duke University, Durham, NC      *              It's Good for you!  |
| tschantz at galactose.mc.duke.edu   *                                  |  



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