membrane protein heterodimer

Dr E. Buxbaum EB15 at le.ac.uk
Mon Sep 22 16:47:30 EST 1997


Christian Ebeling wrote:
> 
> Hello,
> i need for my project membran proteins from any organism which forms a
> specific heterodimer with a strong affinity. This heterodimer should
> also have the property of easy overproduction and purification in
> E.coli. Has anyone an idea?

Na/K-ATPase and H/K-ATPase both are heterodimers of a catalytic alpha-
and a beta-subunit which seems to work as a kind of scaffold for alpha.
These proteins have definetly been expressed in Xenopus oocytes and in
yeast, I am not sure about E. coli (the beta subunit is a glycoprotein).



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