PC12 cells: problems with NGF withdrawal

defaultuser at domain.com defaultuser at domain.com
Mon Sep 29 15:09:46 EST 1997

I'm treating PC12 cells with NGF on collagen coated plates.  After about
a week all of the cells have sprouted neurites... and all's well.

The problem that I'm having is that when I want to wash-out the NGF, the
cells come off of the plates.  I've tried different types of
medium/solutions to do the washes (media w/serum, media w/o serum, PBS),
but no matter what the cells come up.

Any suggestions?  Is this a collagen problem?

Contact me at ssinger at orion.it.luc.edu

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