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help needed on THP-1 transfection

Simon Mauch Simon.Mauch at uni-konstanz.de
Wed Apr 1 11:06:46 EST 1998

Hi everybody,
I need to transfect THP-1 monocytes with plasmid DNA. I tried
electroporation with our BTX ECM 600 using parameters described by
I ve got a huge mass of dead cells (to many for transient analysis)
and no stable transfectants (out of 40 mio cells) after G418

Does anybody have experience with THP.-1 transfection. Any help would
be helpfull (-:


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