SDS-sample buffer

Simon Easterbrook-Smith sbe at biochem.usyd.edu.au
Wed Apr 15 01:50:06 EST 1998


In article <35335839.1DD8A3D2 at mail.uni-mainz.de>, "René Bartz"
<bartr000 at mail.uni-mainz.de> wrote:

Could it be SDS coming out of solution at relatively low temperature? Try
warming the stock solution and see if the flakes go away?




|| Hi!
|| 
|| Since severals weeks, we have a problem with our SDS-sample buffer. Our
|| 1x sample buffer contains:
|| 62.5 mM 0.5 M Tris Cl pH 6,8
|| 10 % Glycerol
|| 2% SDS
|| 0.01% bromphenol blue
|| 
|| The problem now is the development of flakes on the surface of the
|| buffer.
|| Perhaps is there an alternative protocol, which we can use.


Simon
-- 



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