I am a grad student planning to start some nonradioavtive in situ
hybridization experiments in chick embryo sections and whole mounts. As i
decide between biotin and Dig. label i am wondering if there will be a high
background with biotin due to avidin that might be present in the tissue.
I know that it is in the egg white, but is that the only place? We already
have reagents for biotin labeled riboprobes and if I can use them that
would be great. For detection I will use alkaline phosphatase to generate a
If you have experience with chick embryo in situ, or would like to add
your two cents on the biotin -vs- digoxigenin question in general that
would be great. Thank you for your time.