Umbilical cord blood

Doris Anne Morgan dormor at bellatlantic.net
Fri Apr 17 20:49:56 EST 1998


As anyone recovering the CD34+ cells from cord blood knows from
experience, RBC contamination is very frustrating and overwhelming.
Does anyone have an idea as to why the red cells of the neonate do not
pellet in Ficoll? Is it a density difference of the newly formed red
cells? Also I have found them to be quite resistant to hemolysis.
Membrane difference? If anyone is routinely working with cord bloods,
would really enjoy sharing some of these technical problems.




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