nuclear extracts with detergent???

Martin Offterdinger a8803349.nospam at unet.univie.ac.at
Wed Apr 22 03:20:32 EST 1998


Hi!
Currently I am using a method for prepapration of nuclear extracts
that uses dounce homogenisation with a high salt buffer with glycerol
followed by ultracentrifugation. The problem is that my protein of
interest is hydrophobic and I would like to include 0.1% triton-x-100
or NP40 to avoid precipitation of my protein. DO you know if this is
possible without disrupting chromatin structure and releasing genomic
DNA from the nuclei??
Any suggestions welcome!!
Martin
Martin Offterdinger
Internal Med.I,Dept. Oncology
University of Vienna
Austria
E-Mail:a8803349.nospam at unet.univie.ac.at
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