ReRe dissolving IdUrd

Duco dkram at nki.nl
Tue Apr 28 10:23:15 EST 1998


Dear Eugene
Thanks for anwering my question, but this raises another question (as
usual).
Do you have any idea at what pH IdUrd dissolves and until what pH it is
stable? A 25mM TRIS solution, would that be something?

Thanks in advance
Duco


Eugene wrote:

> Hi!
>
> I would use a slightly basic solution to prepare a concentrated stock
and
> then make an appropriate dilution with a growth medium. Works for
BrdU.
>
> Eugene Kandel.
>
> U09577 at uic.edu
>
> Duco wrote:
>
> > In a cell cycle kinetics assay I need to dissolve iodine
deoxyuridine
> > (Sigma I-7125) at 10mM in medium (DMEM/FCS).
> >
> > Does anybody have suggestions?
> > Yours, D. Kramer
> >






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