1998 National Tour Speaker Program.

Victor Benham cw400 at freenet.carleton.ca
Thu Apr 30 23:16:38 EST 1998


SPECTROSCOPY SOCIETY OF CANADA
THE OTTAWA VALLEY SECTION
1998 SAS TOUR SPEAKER DINNER MEETING 

FEATURING

"Prof. R. S. Houk"
Ames Laboratory  - U. S. Department of Energy
Department of Chemistry, Iowa State University,  Ames  IA  50011  USA

PRESENTING
"Identification of Inorganic Elements and Radionuclides in Proteins
and in DNA Fragments by Size Exclusion Chromatography and ICP-MS" 

DATE & TIME: 
Wednesday May 13th 1998. Cash bar (5:30-6:30) pm. Dinner served at
6:30 pm sharp. Presentation scheduled to commence between (7:45-8:00)
pm.

LOCATION:	
The National Press Club, 165 Sparks Street and 150 Wellington Street,
Second floor, Rooms: Library 1 and 2.  Please use Sparks Street
entrance (corner of O'Connor).

PARKING:
Free after 6:00pm on Queen Street or adjacent streets. Also pay
parking at the World Exchange Plaza located between Queen and Albert
Street.

MENU: 
Soup du chef, Roast Sirloin of beef served with a black peppercorn
sauce, New potatoes and Seasonal Vegetables, Apple crumble pie,
followed by Tea or Coffee.

RATES: 
$12.00 Students, $14.00 Members, $16.00 Non-Members. Payment will be
collected at the event. This event is subsidized by the Ottawa
section, SSC.

ABSTRACT:
A general method to identify trace elements in biological molecules is
described. The chromatograms show the distribution of the elements of
interest between protein-bound and free fractions and provide the
approximate molecular weights of the protein-bound fractions.  These
measurements are made in only a few minutes without preliminary
isolation and preconcentration steps.  

Many unusual or difficult elements, such as Cr, Se, Cd, Th and U, can
be observed at ambient levels bound to proteins in human or bovine
serum, milk, and liver extracts.  A new protein, or family of
proteins, that either contain Se or that bind Se molecules, is found
with molecular weight ~ 760 kDa. Alkali metals and Tl are present
primarily as free metal ions and are not bound.   Metal spikes added
to the serum samples can be followed into various compounds.  EDTA
does not remove Fe, Pb, Sn or Th from the proteins but does extract Mn
from some proteins. Procedures for determining the effects of a)
breaking disulfide linkages, and b) replacing essential metals with
toxic ones, on the metal binding characteristics of proteins are also
described.

Binding of metal cations to DNA restriction fragments can be studied
by similar procedures both for essential elements like Mn and Fe and
toxic ones like Cd and Pb.  In particular, trace Pb, Cd and Co are
completely bound to DNA fragments.   Reduction of chromate to a cation
(probably Cr+3) and subsequent binding of the Cr cation to DNA is also
demonstrated.  Binding of uranium, thorium, rare earths (as analogs of
actinides), and ReO4- (as an analog of TcO4-) to DNA is also
described.

RESERVATIONS (space is limited): 
Must be made NO later than Wednesday MORNING, May 13 1998.  Please
contact: 

Victor Benham, Tel/fax: 684 - 4936,  
E-mail:  cw400 at freenet.carleton.ca  

or

Annette Lovett: Tel: 827-5050, fax: 827 - 5011, 
E-mail:  annette.lovett at micromass.ca

NEXT EVENT:
The 1998 Annual General Meeting of Ottawa. 


>>>>>>>PLEASE POST THIS NOTICE.<<<<<<< 


Membership application form is printed below.
.......................................................................................................................................................................................................................


APPLICATION FOR MEMBERSHIP
         
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BOX 332, STN A
OTTAWA, ONTARIO
KIN 8V3

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Student Membership			$20.00

Company Membership		             $85.00





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