CKI, in vitro phophorylation
abigas at SUPERMAC.IRO.ES
Wed Aug 5 13:00:57 EST 1998
I am trying to perform "in vitro phosphorylation" assays with Casein
kinase I and a GST-fusion protein. I am having a great deal of problems
getting my negative control (no enzyme) phophorylated. This also happens
when I use casein as a substrate. If I increase the amount of cold ATP,
all signs of phophorylation disapear.
Does anybody have a protocol for phosphorylation of casein by CKI? or
any other helpful advise for "in vitro phosphorylation". This is a
whole new world to me.
Thank you very much.
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