In article <126.96.36.199.19980805081308.0f47fb14 at email.uc.edu>, holtzcjd at EMAIL.UC.EDU (David Holtzclaw) writes:
>>I was wondering if anyone might have a protocol for growing endothelial
>cells on sylgard (silicone)? Of particular interested is what adhesive
>additive (i.e. fibronectin, gelatin, etc) would work best.
Grow them on sylgard? Good luck!
We grow cells (neural crest) in sylgard wells-- the walls of the well are
sylgard, but the bottom is a tissue culture plastic dish. When you're
ready to look at the cells, you fix and stain them in the wells, then peel
the well of the dish for cover-slipping.
We prepare sheets of sylgard by polymerizing it in petri dishes, then use
a punch to make wells of various sizes. The wells are then cut with a
razor blade, and sterilized by several washes in ethanol. (I've heard
that they can be autoclaved in water, but I've never done that...)
If you're really planning on growing cells *on* sylgard, I'd love to know
how it works.