TUNEL assay

Holger Olbrueck holger.olbrueck at ruhr-uni-bochum.de
Mon Aug 24 08:39:38 EST 1998


Hello,
I have a question concerning the TUNEL assay for apoptosis research. I
try to establish the test (In situ cell death detection kit, Fluorescin,
Boehringer Mannheim) with human BEAS-2B and L-929 cells. After
performing the test, the cells are counterstained with propidium iodide
(PI) and analyzed by fluorescence microscopy. 
I found the cells stained red (PI) and some are stained green
(Fluorescin). Are these the apoptotic cells?

I´m looking forward to your answer.
Holger Olbrueck



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