Transfection of neurons in primary culture

Marek Treiman mat at
Tue Dec 1 10:05:51 EST 1998

Hello netters,

Does anyone have (positive!) experience with transfecting neurons in
primary culture? (The objective is expression of a protein). I would
greatly appreciate some info on the method, conditions, efficiency etc. 
It is generally believed that transient transfection is much more
difficult to obtain in primary culture cells as compared to cell lines.
What are the reasons for this? 

Cheers, Marek

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