Problems With GeneGun-Transfection

Thomas Grunwald grunwald at server3.medizin.uni-leipzig.de
Sun Dec 20 10:51:43 EST 1998


Dear Sir

I need a good protocol for transfection of human cells with a GeneGun.
We have here some persons, who have made many GeneGun-Transfection,
but the efficiency was always under 10%. My idea is, that tey are using
too many goldparticles. There is also a problem with loading the
plastictube,
where the goldparticles are filled in.
I have no idea, where to ask or to search for a good-working protocol
and
which steps are very important and sensitive.

Thanks in advance and merry X-mas and happy 1999
Thomas Grunwald




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