Counterstain needed

Eli Hestermann ehestermann at whoi.edu
Fri Feb 20 10:27:47 EST 1998


Try propidium iodide.  It will stain the nucleic acids in all your cells, and
a 2D dot plot or histogram will allow you to separate your BrdU positive cells
from the rest.

N A Hotchin wrote:

> We are using FITC to detect BrDU incorporation. Can anyone suggest a
> suitable counterstain (detectable by same filters used to detect Texas Red
> and Rhodamine) so that we can easily count non-staining cells. Thanks.
>
> Neil



--
Eli V. Hestermann
ehestermann at whoi.edu
http://www.mit.edu/people/octavian/eli.html
"Vita brevis est, ars longa" - Seneca





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