MTT Protocol

Peter Hohenstein mirihyel at
Wed Feb 25 06:55:09 EST 1998

On 24 Feb 1998, Jon May wrote:

> I would appreciate hearing from anyone who has used an MTT assay to 
> study periosteum and osteoblast viability and proliferation.
> I am having difficulty getting hold of a detailed protocol.
> Thanks
> Jon May

There's a lot info and protocol in:

Loveland et al. Validation of the MTT Dye Assay for enumeration of
Cells in Proliferative and Antiproliferative Assays. Biochememistry
International, 27(3), 1992

Good luck.

Peter Hohenstein
Dept. Human Genetics & Pathology
Leiden University
The Netherlands

tel. (+) 31 71 5276086
fax  (+) 31 71 5276075
E-mail: P.Hohenstein at

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