MTT Protocol

[delios97 at hotmail.com]

Just add the MTT (suspended in RPMI1640 media) to 1/10 the volume of
the culture.  Incubate 1-3 hours at 37 degrees, aspirate media and
lyse in alchol/HCL solution.



On 24 Feb 1998 08:23:04 -0800, JPM595 at NOVELL3.BHAM.AC.UK ("Jon May")
wrote:

>I would appreciate hearing from anyone who has used an MTT assay to 
>study periosteum and osteoblast viability and proliferation.
>I am having difficulty getting hold of a detailed protocol.
>Thanks
>Jon May