MTT Protocol

delios97 at delios97 at
Tue Feb 24 22:32:10 EST 1998

Just add the MTT (suspended in RPMI1640 media) to 1/10 the volume of
the culture.  Incubate 1-3 hours at 37 degrees, aspirate media and
lyse in alchol/HCL solution.

On 24 Feb 1998 08:23:04 -0800, JPM595 at NOVELL3.BHAM.AC.UK ("Jon May")

>I would appreciate hearing from anyone who has used an MTT assay to 
>study periosteum and osteoblast viability and proliferation.
>I am having difficulty getting hold of a detailed protocol.
>Jon May

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