ericbt at umich.edu
Wed Jan 14 11:56:58 EST 1998
I have a question about subsequent transfections. I am using,
primarily, the neomycin gene (G418). I would like to follow this with a
secondary transfection using the psv2gpt gene (mycophenolic acid, etc).
As far as selection medium following the first transfection, I would
ordinarily maintain my transfectants in low dose G418. If I am doing a
second transfection, would my selection medium contain both G418 and the
resistance reagents for psv2gpt and would my final maintenance medium
also contain reagents for both resistances? ( I am not even totally
convinced that the maintenance of stable expression depends on low dose
G418). Any help would be appreciated!
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