Liquid-liquid extraction

Stephen W. Mamber smamber at
Tue Jul 7 17:12:43 EST 1998

Liquid-liquid extraction is no big deal.  I would put one part plasma
and one part ethyl acetate in a large glass test-tube or small flask,
capped with a solvent-resistant (like polypropylene) cap and shaken or
vortexed for say 10-30 minutes.  Then, centrifuge the resulting emulsion
and remove the ethyl acetate layer (which presumably contains your
compound of interest), and dry it down under a gentle stream of nitrogen
gas.  Then you can do your chromatography, etc. on that sample.


Shayne Woodward wrote:
> I need to measure levels of two inactive metabolites of
> chloroacetaldehyde, 2- and 3- dechloroethylifosfamide, respectively.
> The apparent method for measurement is to use a technique called
> liquid-liquid extraction (LLE) using ethyle acetate, then resolving on
> a Chirasil-L-val GC column and then detected via a
> nitrogen-phosphorous-selective flame ionization detector.  Does anyone
> have any knowledge about this or know of a lab or company that could
> run some plasma samples?  Any help is greatly appreciated.  Thanks in
> advance.
> Shayne Woodward
> Surgical Research
> Dartmouth College
> Hanover, NH

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