thin matrigel coating

Krystof kneumann at HOTMAIL.COM
Thu Jul 9 07:44:34 EST 1998


Hi,

Do you have any idea how I could put a .45 micron layer of Matrigel onto
a 12mm Costar micropore insert?  I'd like to not dilute it with serum
free medium if at all possible.  The problem is, the stuff is thick and
it has surface tension.  I can't even get water to do it (just one tiny
bead of water all over the insert).  Any suggestions?  Thanks

Jim Pombrio
pombrioj at envmed.rochester.edu



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