Alternatives to Scraping Flasks of Trypsin/EDTA-sensitive Cells?

Allison Haggarty ar229 at freenet.carleton.ca
Mon Jul 13 10:16:00 EST 1998


PBS citrate works too.  It's just not as fast as trypsin.

Lesley Weston wrote:

> I use trypsin at 0.25% in citrate saline, and it seems to work well without
> damaging the cells. Hope this helps.
>
> Lesley Weston.
>
> On Thu, 9 Jul 1998 da_bruva at hotmail.com wrote:
>
> > .  Can anyone suggest a
> > > less membrane-perturbing alternative?
> > >
> > > Thank you,
> > > Stephen Mamber, Ph.D.
> > > smamber at usccmail.bms.com
> > >
> >
> > Sorry unable to help but...
> > Also very interested...please send me too any suggestions
> >
> > da_bruva at hotmail.com
> > thanks
> > Dave.
> >
> > -----== Posted via Deja News, The Leader in Internet Discussion ==-----
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> >
> >






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