Alternatives to Scraping Flasks of Trypsin/EDTA-sensitive Cells?
ar229 at freenet.carleton.ca
Tue Jul 14 08:07:35 EST 1998
Allison Haggarty wrote:
> Patrick F.H. Lai wrote:
> > Dear Allison,
> > May I have a copy of the recipe of your PBS-citrate, please ?
> > Thank you.
> > Patrick F.H Lai < PFHLai at looksmart.com >
> > Grad. Student, Univ. of Toronto
> The recipe that I used was:
> FW amt
> for 1 litre
> 0.15 M NaCl 58.4 8 g
> 2.7 mM KCl 74.6 0.2 g
> 1.5 mM KH2PO4 136 0.2 g
> 8.1 mM Na2HPO4 142 1.15 g
> 15.5 mM NaCitrate 284.1 4.4 g
Ooops, forgot to add on -- pH is adjusted to 7.2.
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