Alternatives to Scraping Flasks of Trypsin/EDTA-sensitive Cells?

Allison Haggarty ar229 at freenet.carleton.ca
Tue Jul 14 08:07:35 EST 1998



Allison Haggarty wrote:

> Patrick F.H. Lai wrote:
>
> > Dear Allison,
> >
> > May I have a copy of the recipe of your PBS-citrate, please ?
> > Thank you.
> >
> > Patrick F.H Lai  < PFHLai at looksmart.com >
> > Grad. Student, Univ. of Toronto
>
> The recipe that I used was:

>                                                   FW                  amt
>
> for 1 litre
> 0.15 M NaCl                            58.4                          8 g
> 2.7 mM KCl                             74.6                          0.2 g
> 1.5 mM KH2PO4                   136                            0.2 g
> 8.1 mM Na2HPO4                 142                          1.15 g
> 15.5 mM NaCitrate               284.1                       4.4 g


Ooops, forgot to add on  -- pH is adjusted to 7.2.






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