non-toxic fluorescent dye
aeuropa at yahoo.com
Thu Jun 4 23:20:48 EST 1998
Jack Daniel Borgeson wrote:
> I am looking for a non-toxic fluorescent dye which excites around 488 nm
> and emits above 500nm. Any help would be appreciated. Thanks.
What would you need it for? And by non-toxic, do you mean to the cell?
There are a lot of viable stains - for example, Rhodamine 123 stains
mitochondria of live cells, and its excitation is about 485nm;
emission is around 520, I think (I use 510 long pass and 530DF30 with
the confocal microscope).
You can also look into the homepage of Molecular Dynamics. They have a
pretty extensive line of dyes. I talked to their tech support, and
they're pretty helpful, too.
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