p at cam.ac.uk
Fri Jun 5 10:13:46 EST 1998
I always use the Qiagen gel extraction kit to purify linearised vectors, but
the Geneclean kit should work just as well.
How did you test the loss of the 'ligating activity' ?
Was your vector cleaved with one or two enzymes i.e are the ends complementary?
> Hi there:
> I am trying to purify some linearized bands from agarose gels
> with GeneClean ( from Bio101 ) but everytime I try it the
> it4s ligating activity. I have made several trials to ensure
> trouble is with the GeneClean.
> Does anyone use any other way to band purification from
> Any suggestion?
> Yours sincerelly,
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