Course announcement: Microinjection and Detection of Probes in Cells

Rainer Saffrich saffrich at
Mon Mar 16 14:03:28 EST 1998

June 01 - 06, 1998


W. Ansorge, G. Neuhaus, R. Saffrich, M.F. Trendelenburg

Speakers and Instructors:
W. Ansorge (EMBL), H. Andrulat (Hamburg), D. Brocksch (Jena), W.
Blickle (Köln), J. E. Celis (Aarhus)A. Ephrussi (EMBL), R. Fischer
(Heidelberg), T. Hyman (EMBL), R. Kern (Hamburg)I. Mattaj (EMBL), G.
Neuhaus (Freiburg), R. Pepperkok (London), R. Saffrich (EMBL)E.
Stelzer (EMBL), M.F. Trendelenburg (Heidelberg)

The aim of the course is to teach all aspects about capillary
microinjection, automated microinjection, electroporation and particle
transfection techniques 
of cells. The detection and analysis of microinjected and transfected
cells will also be part of the course. Methods and applications will
be discussed and
extensively practised or demonstrated in the laboratory. Lectures and
seminars will complement the course program.
The course is intended mainly for Ph.D. students and postdoctoral
scientists. The selection of participants will be on the basis of
their scientific background, the importance of the course techniques
for their own research and according to the criteria implied by EMBO

 Practical laboratory course program includes i.e.:

· Nuclear and cytoplasmic injection of fluorescent dyes and tracers 
· Nuclear microinjection of DNA 
· Cytoplasmic microinjection of antibodies 
· Injection of plant cells 
· Injection of Xenopus oocytes 
· Injections with an automated system 
· Gene transfer by electroporation into pro- and eucaryotic cells 
· Gene transfer with particle bombardment 
· Detection of injected probes in fixed and living cells 
· Analysis by immunofluorescence techniques 
· Evaluation of experiments and analysis of results by fluorescence
microscopy and image processing 
· Systems for image aquisition and processing

The following applications of the techniques will be discussed: 
DNA transfer, biochemical assays, characterisation of cellular and
nuclear components, fluorescent proteins (BFP,GFP,YFP), Xenopus
oocytes systems, transfer of genes into eggs and embryos, other
methods for transfer of biological material into cells, detection
methods, video and fluorescence microscopy on living systems. There
will be lectures on special topics from invited speakers, blackboard
sessions for the practicals and demonstrations and presentations of
the participants about their own field of interest and research. The
course consists of about 75% practical work and 25% lectures and

Participants may bring their own material (cells, samples, ...) to the
course and attempt to work with it and begin to try solving their own

The deadline for applications is April 1st, 1998. 
Lodging and meals during the course are paid by EMBO. The registration
fee is DM 200,-  but may be waived under special circumstances. The
number of participants is limited to 16

Applications including a short c.v., statement about current research
and recent publications should be sent before April 1st, 1998 to:

Dr. W. Ansorge
EMBL,  Meyerhofstrasse 1		
D-69117 Heidelberg
Fax:      +49-6221-387306
E-Mail:  ansorge at

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